FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10516112
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCACTGTATCTCGTATGCCGT113440.10787256735188822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG17850.041.673371
TTGACGG22700.030.1255931
GGTACGA12650.026.08474318
TGACGGC26750.025.7887732
CGGCTAT27500.024.64940535
TAGGACG45750.024.522394
GTACGAG13500.023.99899119
CGTACGT12900.023.71564510
AACTGCG13600.023.3791477
CCGTACG13550.022.5797149
TGGTACG15000.022.39799117
TACGAGG15450.022.13541220
CTCGAAT15850.021.95094512
TTTACGC7150.021.8162253
GCCGTTA17350.021.78448116
GCGTTTA12750.021.64254635
AATCACG35700.021.5081234
CTACGGT11750.021.44203213
ACGGCTA30100.021.32445534
TAGTGCG16200.021.1064128
Produced by FastQC (version 0.11.2)