FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10503711
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG16100.035.40921
TACGCCG9050.027.184325
TTGACGG19900.026.84279331
TCTCGGG33200.024.939104120
TAGGACG42150.024.34544
TTTACGC8650.022.8937133
CGGTGGG39600.022.878183120
GTTCGCA16350.022.3836659
CGCCGTT18200.021.428007120
GACGAGG53200.021.202448120
TTTTACG10550.021.0459062
TGACGGC27200.020.96261432
TCGCCGG54100.020.849726120
TCGACTA15750.020.19696429
CGTATCC108750.020.192572120
CGTGATT35800.020.11819328
CCGACGG27150.020.109966120
CGGCTAT27300.019.78054635
CCGTGAT36350.019.64867827
GTCCTAA53550.019.4988141
Produced by FastQC (version 0.11.2)