FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10503711
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCACTGTATCTCGTATGCCGT112200.10681938983279338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG18300.042.2872771
TTGACGG22500.030.659431
CTACGGT11750.026.54563713
TGACGGC29050.024.98547432
CGTACAC10400.024.2298723
TACGGTG12950.024.08569314
GGTACGA14550.023.50070618
CGGCTAT28650.023.2411235
TGGTACG15250.022.42198417
TAGGACG47600.021.9319634
CGTCTAA14550.021.439341
TCCGCAA14000.021.42349633
ACGGCTA29550.021.31525434
TCTTACG15600.021.15004244
TACGTAC8300.020.9599861
TAGTGCG13450.020.51431528
GTCCGCA15800.020.5014732
GGACGTG51400.020.3082126
CCTACGG15700.020.24901412
CGCAATT12750.020.23021149
Produced by FastQC (version 0.11.2)