FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10523086
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG31800.027.545572120
CGTCTAG17750.027.0459561
TCGCCGG50500.025.305462120
CGCCGTT19050.025.19539120
TAGGACG45850.023.1647764
CGGTGGG41950.022.454002120
TGGTACG19200.022.18695317
GGTACGA19100.021.6746518
TTGACGG19400.021.34485231
CGTATCC103550.021.263855120
TATGGCG54450.020.82593216
ATTCGAA14750.020.75027832
TAGTGCG12500.020.64449528
GCGTTTA13100.020.61164735
GGACGTG50250.020.5389546
CGAATTG15150.020.19867122
GTATAGG42350.019.9791221
GTCCTAC79550.019.763861
TTCGAAG15850.019.68882833
TATTACG8250.019.63727836
Produced by FastQC (version 0.11.2)