FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10523086
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCACTGTATCTCGTATGCCGT112860.10724990748911488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG16550.039.8732531
TTGACGG21200.028.86077331
TAGGACG43450.024.9910244
TACGTAC8700.024.1343611
TGACGGC26750.023.99405332
CGTACAC11150.023.6740783
CGGCTAT26750.022.87283535
ACGTACA11350.022.728242
TATGGCG56750.022.19872516
GGTCGGT28050.021.38700511
CTACGGT13550.021.2507613
GGACGTG48950.020.957766
TGGCGAG61400.020.71295518
ACGATTT14200.020.69909730
ATCGCTA11350.020.61163327
CGTGATT32700.019.99504728
GTCCTAC79150.019.933881
ATTTACG22350.019.86094526
GCGTTTA11850.019.74194535
TATTACG8550.019.64462936
Produced by FastQC (version 0.11.2)