FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10464688
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG15050.032.2995761
TCTCGGG30200.029.400991120
TAGGACG44700.023.7631324
GCGTTTA11500.022.4381935
GGTACGA16600.022.40961318
GTCCTAC76500.022.1225911
CGCCGTT18550.021.992378120
TCGCCGG54050.021.755426120
TTGACGG19750.021.27057631
CGTATCC110900.021.152044120
CGTCATT29150.020.99262446
GACGAGG53750.020.872301120
CCGACGG25100.020.794703120
GGACGTG50900.020.7494146
CGGTGGG40600.020.687557120
GTACGAG17850.020.50525719
TGGTACG18100.020.22097217
CGTATGA10400.020.1968212
TACAGTG91800.019.8078735
AGGACGT59250.019.6494925
Produced by FastQC (version 0.11.2)