FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10464688
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCACTGTATCTCGTATGCCGT113980.10891867965867688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG14800.039.3211751
TTGACGG18100.028.83236931
TAGGACG45350.025.5321944
GCGTTTA13500.023.10517335
TGACGGC23500.022.71756732
CGGCTAT23750.022.47843435
CTACGGT12800.022.0251713
GTATAGG41550.021.514521
GGACGTG51100.021.3679456
GTAGGAC80050.021.2100433
TAACGTT12150.020.73798816
TACGGTG14200.020.70147714
TATGGCG56800.020.27916
TAGTGCG13350.020.2196228
CGTCTAA12850.020.0762121
TAGTCGA16750.020.057715
AATCACG33500.020.05473934
CTAACGT12600.019.99734715
TACGTAC6350.019.8409291
TCCGCAA13350.019.770233
Produced by FastQC (version 0.11.2)