FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10457126
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG13900.033.6792341
TCTCGGG32050.027.703016120
CCGACGG24850.024.141651120
CGGTGGG39700.023.422573120
TCGCCGG51950.023.327015120
CGCCGTT18750.023.036932120
TAGGACG43800.022.609354
TACCGTA14300.022.2383087
TTGACGG19500.021.54403731
GGTCGGT25700.021.24311611
TATTACG8200.021.22358536
GCGTTTA11900.021.1815535
GACGAGG49650.021.024387120
TCACGTT31500.020.95720727
GTCCTAC79150.020.4736481
GTATAGG37950.020.4014171
CTACAGT93650.019.931084
TACAGTG96350.019.6834455
CGTGATT34500.019.6572628
ATACCGT16200.019.6326476
Produced by FastQC (version 0.11.2)