FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10457126
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCACTGTATCTCGTATGCCGT110820.105975580671018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG16250.037.2865831
TTGACGG20700.026.6596431
CGTACAC10550.023.8824753
TAGGACG44850.023.8089394
TACGTAC8600.022.3221531
CGGCTAT23900.022.08612435
GTCCTAC75400.022.0390571
CGCTATA11750.021.95124229
ATCGCTA12300.021.94512227
GGTACGA15650.021.46409618
TGACGGC26650.020.9325732
ACGTACA10850.020.4576872
AGTCGGT15600.020.37934317
GGACGTG52350.020.2807046
TACTCCG21050.020.2343255
CTACGGT11950.020.07954213
TATGGCG57400.020.06564516
TACAGTG91900.020.0403545
CTACAGT87250.020.0082954
CGGTTGT16850.019.93511420
Produced by FastQC (version 0.11.2)