FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10470689
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG14900.030.610271
TCTCGGG32100.027.100695120
TTGACGG16650.027.03008731
CGCCGTT19750.023.086811120
TCGCCGG52700.022.996311120
TAGGACG43350.022.5651554
CGGTGGG42450.021.906422120
CCGACGG28300.021.835756120
AACTGCG14600.021.7801677
CGTGATT35000.020.91605428
GTCCTAC80250.020.4901621
CCGTGAT34950.020.43091227
CGTATCC114050.020.252697120
TATTACG8000.020.25103636
TGACGGC22950.020.13290232
TAGTGCG14200.019.86083428
GCTCGAA16450.019.32961711
GGTACGA15850.019.3057818
CGTACGT14350.019.23176810
GGACGTG47600.019.161016
Produced by FastQC (version 0.11.2)