FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10470689
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCACTGTATCTCGTATGCCGT111750.10672650099721231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG15050.036.2756161
TTGACGG19100.025.44053831
GGTACGA16550.024.2850518
TAGGACG47950.023.3954724
GTACGAG17900.022.45349519
TGGTACG17700.022.368417
TACGCCG8950.022.1191085
TTGGACG15750.021.70993617
TACGAGG18950.021.2092720
GGACGTG52750.020.9233196
CGTACAC10900.020.9139423
TTTACGC7950.020.3739593
CGTACGT14750.020.33539434
CGTAATC15350.020.32127422
TACGTAC8000.020.2480721
GCGTAAT7750.020.1271151
TGACGGC25350.020.11479632
TAGTCGA17900.020.108285
CTCGAAT14650.020.06404712
CGTAATA7500.019.9980952
Produced by FastQC (version 0.11.2)