FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10496588
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGT16800.054.2058893
CGACGTA22900.053.4180392
TACGAGT11300.050.51864694
TCGACGT24800.049.87385691
ACGTATC24450.048.36389594
ACGCCGT19900.047.8107894
CACCGTA24500.047.43303393
GTCGGTG19650.046.34396494
ATGTCGG21000.045.9536492
ATGGTCG15100.044.1062494
GGTCGAC27850.043.9248289
TCGGTAG13500.042.28713289
TAGATCG18550.042.13289688
GTCGACG29550.041.62692390
GTGGACG25350.041.2853590
GTGATCG16300.041.27630691
ACCGTAT28200.040.96855594
CTACGAG14150.040.82379593
TGGTCGA29800.040.82293388
TAGTCGC13550.040.625393
Produced by FastQC (version 0.11.2)