FastQCFastQC Report
Mon 11 Dec 2017
PWK-M-frozen-3-library_ACCACTGT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-M-frozen-3-library_ACCACTGT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10496588
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCACTGTATCTCGTATGCCGT110110.10490075441657803No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG15500.039.0947951
ATTACGC8000.030.7465437
TTGACGG19750.026.42526631
TATTACG8550.025.9606336
CTACGGT11950.025.09998513
TACGGTG12100.023.79682214
CGTACAC10400.023.6524243
TACGTAC7950.023.3950441
TAGGACG48200.023.1523554
CGTAATA8050.022.3591182
CGATCAA16550.022.11041830
CGTATCG11850.021.76804410
GCGTAAT8600.021.6268121
ATTACCG12900.021.39110629
ACGATCA16900.021.29755229
GGACGTG53100.021.1256546
CGGCTAT25300.020.86552835
AACTGCG15550.020.832187
GTAGGAC84750.020.3881553
GGTACGA15750.020.18762618
Produced by FastQC (version 0.11.2)