FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16854376
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT543360.3223851182624619Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG24400.030.9888361
CGCCGTT20100.026.269115120
TTGACGG30750.023.80621131
CGTATCC103800.023.468618120
CTACGGT20250.023.40580213
TCGCCGG52650.022.108648120
TACGGTG21450.022.09638614
TCTCGGG37800.022.063875120
CGGTGGG49500.020.848846120
TGTCGAA29250.020.51153419
ATTCGAA27950.020.39470332
CGAATTG28700.020.27837822
CCGACGG31350.019.713263120
TCCGCAA24150.019.3799133
TTCGAAG29400.018.98059833
CTAGACG25450.018.8636933
TGACGGC40150.018.68099632
CCTACGG25400.018.66013512
GTCCGCA26250.018.51532232
ATGTCGA30200.018.07828318
Produced by FastQC (version 0.11.2)