FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16854376
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTA514900.3054992958505257No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTATGCCGT222230.13185299770219913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG22350.033.2817421
TACGGTG22200.025.12862414
TTGACGG29250.024.60929931
CTACGGT22950.024.04605513
TCCGCAA24350.021.92476333
CTAGACG28350.020.3148483
GTCCGCA26700.020.21971932
CGCAATT29150.019.34312849
AACTGCG32000.019.1215217
TGACGGC37750.019.06813232
CCTACGG28950.018.85527212
TAGGACG44550.018.7180774
TGGTACG27650.018.44143517
TCTTACG32250.018.22783544
CATCGTT27000.018.21771632
TAGACGC74100.018.1352674
GGTACGA27900.018.0609618
ACGCACC30900.018.0549667
ACGGTGA31150.017.90910715
TGCGACC28900.017.85004610
Produced by FastQC (version 0.11.2)