FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16829315
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT541380.3216886724147715Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG24100.031.1241051
CTACGGT20550.024.5244413
TTGACGG30650.024.08000631
CGCCGTT21750.023.449099120
TACGGTG21450.022.65631914
CCGACGG30050.021.76449120
TCTCGGG36700.021.581139120
TCGCCGG55900.021.467657120
CGGTGGG50450.020.694483120
TCCGCAA23450.020.2146533
CGTATCC106600.020.207087120
GTCCGCA25000.019.9214132
CTAGACG27450.019.8931223
TGACGGC37550.019.65518432
TGACGCC41400.019.42097120
CCTACGG25700.019.14310512
GGTCGGT52500.018.85629311
TAGGACG42500.018.7787484
ATATCGT23200.018.102921
ACGGTGA30950.017.83441215
Produced by FastQC (version 0.11.2)