FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16829315
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTA513950.30538973214298976No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTATGCCGT218780.1299993493496319No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG24900.037.1022031
TTGACGG30850.027.61139331
CTACGGT23900.023.09064313
TGACGGC37750.022.72344832
TACGGTG24550.022.4792814
TCCGCAA25650.021.28183733
CTAGACG30500.019.8672893
CCTACGG28550.019.53992712
CGGCTAT47700.019.24119435
GTCCGCA28750.019.19575132
GGTACGA27100.019.03767818
GGTCGGT50150.018.89871411
CTTGACG47200.018.42823430
ACGGCTA47550.018.41890734
GTACGAG28450.018.13495419
TAGGACG40300.018.013474
ACGCACC31750.017.9500087
AACTGCG33300.017.8351087
TGGTCGG31700.017.5982610
TGCGACC28650.017.58736410
Produced by FastQC (version 0.11.2)