FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16824750
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT533860.31730634927710666Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG26000.032.3120351
TTGACGG28950.026.1205331
CGCCGTT18850.023.872766120
TCTCGGG37100.023.450222120
CTACGGT20100.022.3868113
CCGACGG29150.021.818262120
TACGGTG21550.020.88050714
TGACGGC37500.020.80520832
CGGTGGG49000.020.571505120
CGTATCC108600.020.55256120
GGTCGGT49600.020.44240811
TCGCCGG51850.019.672203120
TGACGCC40050.019.1761120
TCCGCAA23900.018.5814533
CGAATTG28150.018.11729622
CGGCTAT48150.018.06854835
CGTCTTA24600.018.04777315
ATTCGAA28800.017.92115232
TAGGACG38350.017.8381214
GCGTTTA30300.017.82179535
Produced by FastQC (version 0.11.2)