FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16824750
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTA506080.3007949598062378No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTATGCCGT213370.12681912064072273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG24700.035.9462551
CTACGGT22750.025.8398313
TTGACGG32100.025.78900131
TACGGTG23250.025.02620914
CCTACGG25450.023.56987212
TCCGCAA26000.022.37993833
TGACGGC39750.021.12765932
GTCCGCA29150.020.16730932
ACGCACC30150.019.4993957
CTAGACG31800.019.0555653
TAGGACG40850.018.7994654
GGTACGA25800.018.60164618
TAGACGC76500.018.5872214
ACGGTGA32800.018.28826115
TGGTACG26600.018.042217
ATCGCTA25300.018.01930651
CGCAATT29100.017.93402149
ATTCGAA28500.017.89099532
GTACGAG27600.017.8255419
CGTACGT24800.017.65691810
Produced by FastQC (version 0.11.2)