FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16804642
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT534810.31825134983536096Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG24750.025.9429151
TCGCCGG51100.023.716549120
CTACGGT21900.023.28674513
CGCCGTT20350.022.995943120
TACGGTG22250.022.65078214
CGTATCC106650.022.50195120
TCTCGGG35850.022.425217120
CCGACGG32800.021.583864120
GGTACGA26350.021.40325718
ATTCGAA29000.021.31583832
CGAATTG28550.020.80644822
TTCGAAG30250.020.63341533
TTGACGG29250.020.51810831
GTACGAG27550.020.25490419
TGGTACG27900.019.99920317
TCCGCAA24050.019.96356433
CCTACGG25100.019.60081112
ATGTCGA30400.019.34127618
TACGAGG29950.019.23283220
TGTCGAA31750.019.08738719
Produced by FastQC (version 0.11.2)