FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16804642
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTA507490.3019939371514133No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTATGCCGT218800.1302021191525532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG26250.034.510041
TCGTACG3550.032.1088297
TTGACGG31000.028.0569731
CTACGGT23250.025.8003613
TACGGTG24350.024.63535314
TCCGCAA25550.023.24221433
CCTACGG27700.022.52182612
TGACGGC39350.022.2557732
CTAGACG31000.020.5141543
GTCCGCA29100.020.40688332
TAGTGCG24350.019.95356828
ACGCACC31550.019.7749337
TTAGACG31050.019.3218143
TAGACGC78000.019.1520024
ACGGCTA45550.019.094734
CGGCTAT49300.018.73735635
GGTCGGT50600.018.6131511
ACGGTGA34200.018.41708815
CGTACGC6300.018.09306598
AACTGCG32500.017.9047977
Produced by FastQC (version 0.11.2)