FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16764269
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT534860.31904761251444963Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG24800.033.1526151
CGCCGTT22150.026.815027120
TCTCGGG37700.026.098724120
TTGACGG31850.025.62639231
TCGCCGG49750.024.48047120
CCGACGG31350.022.007824120
CGTATCC110250.021.277224120
ATTCGAA28900.020.35102332
CTACGGT21000.020.28483213
CGGTGGG46400.020.170815120
CGAATTG29350.019.83037622
GGTACGA26300.019.61950318
TGACGGC41650.019.59665332
TGGTACG27400.019.26980817
TTCGAAG30850.019.25919233
TACGGTG21450.019.02071814
TGACGCC43950.018.838097120
GGTCGGT51900.018.72717311
CGGCTAT49400.018.5856835
GTACGAG27800.018.56183819
Produced by FastQC (version 0.11.2)