FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16764269
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTA511090.3048686465243429No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTATGCCGT213370.12727665011817693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG23250.038.1897851
TACGGTG23200.029.73712214
CTACGGT23500.028.8437513
TTGACGG30100.027.50149531
TCCGCAA26150.025.92084733
CCTACGG27450.024.91364912
CTAGACG29450.024.0383823
GTCCGCA29950.022.63205732
ACGCACC32350.021.3252037
TAGGACG38400.021.2476544
TGACGGC39500.021.10863332
ACGGTGA33700.020.82787715
TAGACGC78700.020.4297664
GACGCAC50450.020.3346616
GCGTTTA27150.020.10563935
CGGCTAT48300.019.49839235
TACGTCG5750.018.77773585
GACGCTT32650.018.374646
CGCAATT28850.018.29735649
TAGTGCG26650.018.00690728
Produced by FastQC (version 0.11.2)