FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16743194
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT532860.3182546890396181Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG25100.030.845571
CGCCGTT21600.023.885735120
TTGACGG29750.023.40039331
TCGCCGG51850.022.44641120
CTACGGT21650.022.1695613
CCGACGG31950.021.593391120
TACGGTG22150.021.12922114
CGTATCC116200.019.722008120
TAGGACG40350.019.4848374
CGTCTTA23700.019.2412415
TGACGCC45750.019.145012120
CGGTGGG50000.019.077478120
TAGACGC71800.019.058094
GGTCGGT51000.018.7042511
TCCGCAA24450.018.65463833
CGAATTG26000.018.46268322
TCTCGGG35650.018.342598120
GGTACGA25550.018.3177818
CGCAATT29600.018.24083549
TGACGGC38850.018.22817232
Produced by FastQC (version 0.11.2)