FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16743194
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTA508720.3038368903806526No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTATGCCGT216960.129581010648267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG24650.034.55791
CTACGGT21200.026.88060413
TACGGTG22700.025.89727814
TTGACGG30800.024.3446131
CCTACGG25300.022.52425812
TCCGCAA24250.022.01515433
GTCCGCA27250.020.47198332
TGACGGC38200.019.94269832
CTAGACG29800.019.7277683
GGTACGA29150.019.54871418
TGGTACG29800.019.32360317
ACGCACC29550.019.2834637
GCGTTTA29350.019.2115335
ACGGTGA31000.018.5760915
GTACGAG30850.018.47163819
TAGTGCG23750.018.4366528
TACGAGG32100.018.12580120
GACGCAC51100.017.6072626
CGCAATT27950.017.5977649
TAGGACG40700.017.539644
Produced by FastQC (version 0.11.2)