FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16751811
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT532270.3177387806010944Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG24800.031.215791
TTGACGG28050.025.45679331
CTACGGT20500.024.29120313
TACGGTG20200.024.05865914
TCGCCGG50700.022.010035120
TCCGCAA22650.021.72377433
CGCCGTT20950.021.477957120
TGACGGC35550.021.0989132
TCTCGGG37100.021.02254120
CGTATCC115150.020.632269120
CCTACGG24350.020.45001212
CTAGACG26800.020.3771233
CGGTGGG47600.019.536219120
CCGACGG30100.019.333967120
GTCCGCA25700.019.1456632
GGTCGGT52550.018.83754711
CGCAATT27600.018.25937749
CGAATTG27450.018.14221822
TAGACGC72600.018.1027184
GCGTTTA30700.017.5910835
Produced by FastQC (version 0.11.2)