FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16751811
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTA509780.3043133664772125No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTATGCCGT214510.12805182675473117No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG23450.036.0725361
TTGACGG30200.027.41261931
CTACGGT23700.025.31251113
TACGGTG24650.024.82371914
TCCGCAA23900.024.34737833
TGACGGC37100.022.7994132
GTCCGCA26400.022.26899132
CCTACGG27850.021.54030612
CTAGACG29700.019.9961683
ACGCACC29900.019.6601987
GGTACGA26600.019.61953718
TAGACGC73750.019.277664
CGGCTAT50350.019.18253935
TGGTACG27250.018.93175517
TAGGACG41450.018.5247964
TTAGACG30500.018.488263
GTACGAG28450.018.34380519
CGAATTG26950.017.80702822
ACGGTGA34200.017.71542515
ACGGCTA49250.017.54020334
Produced by FastQC (version 0.11.2)