FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16804490
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT525960.3129877788614828Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA25350.049.4039292
TCGACGT26200.048.0609191
TGTCGGT19900.046.51653793
TAGATCG21750.045.06593788
ACGTATC28300.042.81097494
GTGATCG17700.042.684691
ACGCCGT20500.041.50282794
GGTCGAC30750.041.17164289
TGGTCGA32050.039.7153888
TGATCGC19400.039.64589392
GATCGCG24850.039.44201790
GTCGACG32350.039.1346790
TACGAGT16300.038.83442394
ATCGCCG19050.037.5159494
TGCCGAT19350.037.28808288
ATGGTCG15150.036.3909994
GTCGATC21500.035.7735192
GTCGGTG25250.035.58230694
AGATCGC27650.035.44849489
CACCGTA31250.034.849193
Produced by FastQC (version 0.11.2)