FastQCFastQC Report
Mon 11 Dec 2017
PWK-F-ATAC-2-13_GTCGTGAT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePWK-F-ATAC-2-13_GTCGTGAT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16804490
Sequences flagged as poor quality0
Sequence length126
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTA499310.29712892209165526No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTATGCCGT209160.1244667347833823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG24550.040.8111461
TTGACGG30550.030.63255531
TGACGGC37500.025.43515632
CTACGGT23000.024.5170713
TACGGTG24350.022.91076714
TCCGCAA26050.021.41620333
CCTACGG26800.021.0407712
TAGACGC72350.020.7312534
TTAGACG28850.020.171453
GGTACGA26500.020.14770518
ACGCACC29000.020.0658027
CTAGACG31000.019.9336453
CGGCTAT49150.019.89495535
GTCCGCA27950.019.7457932
GACGCAC48450.019.1914486
GTACGAG28450.019.18859519
TACGAGG28950.019.06440720
TAGGACG40800.018.9694024
TGGTACG28050.018.82050517
CTTGACG50300.018.7240730
Produced by FastQC (version 0.11.2)