FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13402493
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT348820.2602650118899521Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT26950.020.705248120
TAGGACG109000.020.204864
GTCCTAC126900.019.3409561
CGTATCC105550.019.213867120
GACGAGG60000.019.100222120
TCTCGGG45100.018.891573120
CGTCTAG15500.018.5834261
TCGCCGG61900.018.513948120
GGACGTG116700.017.737076
AGGACGT124300.017.7164695
TACAGTG161200.016.7877985
GTAGGAC127350.016.680943
CTACAGT158800.016.4760194
CCGACGG36250.016.055359120
CGGTGGG55050.015.695005120
CCTACAG157200.015.574853
TAGATCC127000.015.4962435120
GTCCTAA106850.014.9390631
GACGTGG70350.014.9245437
TGTAGGA163250.014.8873732
Produced by FastQC (version 0.11.2)