FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13402493
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT349960.26111559991115085No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA326990.24397699741383938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG110350.022.2901884
CGTCTAG14550.021.0268271
GGACGTG119550.019.7712556
AGGACGT127100.019.4942575
GTCCTAC120650.018.9933951
GTAGGAC134500.018.4663163
TATGGCG118600.017.45035216
TACAGTG156000.016.8057945
CTACAGT153750.016.5444724
ATATGGC233650.016.3546115
TGGCGAG131750.016.02759418
CCTACAG153400.015.9564773
TCCTACA160050.015.8171482
AATATGG252600.015.74414914
ATGGCGA135950.015.53244217
GACGTGG76300.015.41006857
TGTAGGA174050.015.4065332
GGTACGA10150.015.36680418
CTGTAGG167300.015.2749651
TACACTG112450.015.2583935
Produced by FastQC (version 0.11.2)