FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13488871
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT349850.2593619584619054Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC110950.020.712324120
GTCCTAC123600.020.5851041
TCTCGGG48850.019.529446120
TAGGACG106550.019.4862524
CCGACGG38000.018.789738120
GACGAGG63650.018.570568120
TACAGTG159450.018.0248475
GGACGTG114600.018.0097246
TCGCCGG66750.017.887892120
CGTCTAG14800.017.8400751
GTAGGAC125200.017.6379933
CTACAGT157100.017.3414484
CGGTGGG58150.017.334724120
AGGACGT122200.017.2344115
TAGATCC133450.016.500793120
TATGGCG113500.016.386816
GACGTGG71250.016.2517767
CGCCGTT25250.016.158644120
CCTACAG155750.016.1432743
TCCTACA165500.016.0624372
Produced by FastQC (version 0.11.2)