FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13488871
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT353600.2621420280466764No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA328390.24345254691812238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG107300.021.751014
GTCCTAC119100.020.650971
GTAGGAC127100.019.2594623
GGACGTG114450.019.1854656
AGGACGT123450.018.8568275
GACGTGG73000.017.1760797
TACAGTG158150.016.8059275
TGTAGGA167450.016.4077382
CGTGGAA75400.016.3906619
GCGTAAT9150.016.3903141
CTACAGT157900.016.224654
TCCTACA161550.016.0415082
TACACCG16200.015.9250725
ACGTGGA76700.015.8781998
CGTCTAG13250.015.8460321
CCTACAG156050.015.8402863
TATGGCG118650.015.36978516
AGTGGAC104350.015.2928978
CTATACT72250.015.2795144
CTGTAGG161550.015.2617121
Produced by FastQC (version 0.11.2)