FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13504975
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT349320.2586602344691493Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC112150.019.902023120
TCGCCGG65200.019.693357120
TAGGACG108950.019.3877284
TCTCGGG47300.018.900736120
GTCCTAC122650.018.1517221
TATGGCG117100.017.52241116
CGGTGGG56900.017.39904120
GGACGTG118150.017.3676556
AGGACGT123750.017.2130415
CGTCTAG17800.016.856271
GTAGGAC127500.016.378743
CGCCGTT24200.016.363724120
CCGACGG39350.016.315208120
TGGCGAG131400.015.98081618
ATGGCGA135500.015.76287217
TACAGTG156550.015.4080425
ATATGGC227200.015.395184515
TAGATCC135800.015.243087120
CTACAGT150750.014.847834
GTCCTAA107400.014.8065221
Produced by FastQC (version 0.11.2)