FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13504975
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT349970.2591415385811525No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA329690.24412485028665362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC121750.021.7786521
TAGGACG106950.020.9795344
GGACGTG115250.018.7901866
TACAGTG165700.018.5736925
GTAGGAC128200.018.4847723
CTACAGT157750.018.4449464
AGGACGT123350.018.0928615
CGTCTAG14300.018.0389021
TCCTACA161050.017.8800142
CCTACAG157550.017.0975153
CTGTAGG159650.015.8194321
TTCCTCG87800.015.78302943
AGTGGAC106200.015.7032058
TATGGCG117800.015.429008516
TGTAGGA167550.015.32451252
GACGTGG71550.015.0914497
GGACATT114900.014.87871911
ATATGGC225800.014.85007315
TACACTG116500.014.7279915
TCCTCGC87000.014.54909844
Produced by FastQC (version 0.11.2)