FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13406334
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT341090.25442451306971764Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG47000.021.190289120
TCGCCGG63900.020.562214120
CCGACGG37750.020.343218120
TAGGACG106300.020.3215794
CGCCGTT26400.019.771606120
CGTATCC105800.019.564108120
GTCCTAC129800.019.2780251
CGTCTAG15500.018.195641
GGACGTG114000.017.7905886
AGGACGT121150.017.5830065
GTAGGAC123650.017.373613
TAGATCC130700.017.305828120
TACAGTG161550.016.78885
CGTGATT74400.016.37443728
CGGTGGG55250.016.288671120
GACGAGG62800.016.241117120
TATGGCG117750.016.10135816
CTACAGT158050.016.0595744
TCCTACA168250.015.9423952
CCGTGAT74300.015.66953827
Produced by FastQC (version 0.11.2)