FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13406334
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT348540.2599815878076736No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA320560.2391108561072699No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG116800.021.6239094
GTCCTAC120050.019.8888361
GTAGGAC136950.019.5812513
GGACGTG122950.019.175116
AGGACGT130650.018.8733235
GACGTGG78700.017.2265957
TGTAGGA174550.016.4972462
TATGGCG123550.016.45979316
CTGTAGG169150.016.4209181
ATATGGC235700.016.39055415
TACAGTG162850.015.91519455
CGTCTAG14800.015.8085251
TCCTACA162400.015.773642
CTACAGT154300.015.6687394
TATGGCA119400.015.62512316
CCGTGAT72600.015.61565527
CGTGATT72300.015.59748428
AATATGG258500.015.40901714
CGTGGAA83150.015.3667329
GTCCTAA106800.015.3348611
Produced by FastQC (version 0.11.2)