FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13479022
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT352190.26128750290636815Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC126000.020.0515021
TAGGACG110450.019.9402644
CGTATCC118300.019.778622120
CCGACGG35400.019.659428120
CGCCGTT26300.019.618187120
TAGATCC140300.018.430443120
TCTCGGG45600.018.287996120
TCGCCGG65700.018.263365120
TACAGTG160400.017.8461515
GGACGTG117150.017.4671366
CTACAGT152250.017.42194
AGGACGT126400.017.2816355
GACGAGG62000.017.127647120
AGTGGAC104400.016.4366138
CGGTGGG57300.016.33376120
GTAGGAC130150.016.1383553
TCCTACA166000.016.0874542
CCTACAG156350.015.621833
TATGGCG121450.015.36424616
TGACGCC51150.014.896156120
Produced by FastQC (version 0.11.2)