FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13479022
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT350320.2599001618960189No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA333350.24731022770049638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC125350.022.0157241
TAGGACG109600.021.9497724
CGTCTAG17600.021.4747121
GTAGGAC135150.019.975423
GGACGTG118900.019.4763746
AGGACGT128500.019.048175
TACAGTG162650.018.7372515
CTACAGT159250.018.233174
TCCTACA165900.017.791732
CCTACAG159100.017.6848813
TGTAGGA169200.017.4447272
GACGTGG76400.016.8817397
CTGTAGG164050.016.7124231
ATATGGC226800.016.50578515
TATGGCG119350.016.38663716
AGTGGAC104900.016.2982658
TTCCTCG89950.015.80584143
AATATGG247850.015.66065714
TCCTCGC86900.015.60100844
ACGTGGA80050.015.5874138
Produced by FastQC (version 0.11.2)