FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13450175
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT346380.25752824777372785Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT25200.021.188131120
TCTCGGG45550.020.809992120
TAGGACG105800.020.4213644
GTCCTAC126700.019.326781
CCGACGG37400.019.249207120
CGTCTAG13950.018.9301661
CGTATCC121150.018.916603120
GGACGTG112000.018.5380736
AGGACGT119300.018.3619465
CGGTGGG54800.018.282648120
TCGCCGG65300.016.904715120
GTAGGAC126150.016.794363
TACAGTG162900.016.4316065
CTACAGT158450.016.3628674
TCCTACA170250.016.2866152
TAGATCC148050.015.803722120
AGTGGAC105650.015.7876418
TGACGCC51300.015.67078120
TATGGCG114650.015.59565116
GACGAGG58100.015.488819120
Produced by FastQC (version 0.11.2)