FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13450175
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT351900.2616322835948231No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA329700.24512692214041826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG13750.022.2505531
TAGGACG111900.021.7654514
GTAGGAC135600.020.527143
GGACGTG123050.019.3521396
AGGACGT128850.019.2747025
GTCCTAC122250.019.1376721
CGTGGAA77650.017.6896389
GACGTGG78100.017.6643187
TGTAGGA174500.017.120142
ACGTGGA81650.017.0432918
CTGTAGG170250.016.878031
ATATGGC226000.016.80179815
TATGGCG117450.016.65047516
AATATGG247650.016.08391614
TACAGTG158400.016.0197945
TATGGCA118000.015.91198416
TCCTACA160100.015.8122792
CGACTTG69500.015.74189564
CTACAGT151200.015.7114094
TTGACGA73200.015.4052460
Produced by FastQC (version 0.11.2)