FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13365004
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT336390.25169464969857097Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG106800.021.0154554
TCTCGGG43200.020.69279120
GTCCTAC126550.020.343871
CGCCGTT26800.019.923782120
CGTATCC122550.019.778101120
CGGTGGG57450.018.588593120
AGGACGT123650.018.5399115
GGACGTG113600.018.22316
GTAGGAC124900.017.6336443
TCGCCGG63650.017.531984120
TACAGTG161400.016.7319815
CTACAGT159050.016.6018854
TCCTACA166350.015.945492
TAGATCC153150.015.904703120
GACGAGG60150.015.859081120
CCGACGG33450.015.783492120
CGTGATT72300.015.68597528
CCGTGAT74600.015.60453927
AGTGGAC107100.015.4052198
GTCCTAA111950.015.3849531
Produced by FastQC (version 0.11.2)