FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13365004
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT344030.257411071481909No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA319330.23892996964310675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG109550.022.3967934
GTCCTAC120300.021.2447381
AGGACGT125650.019.8612125
GGACGTG121100.019.3671286
GTAGGAC133600.017.3771863
CTACAGT153450.017.3576534
TACAGTG160250.017.2574985
CCTACAG151600.017.2133333
TCCTACA163550.017.204012
CGACTTG67200.016.36169664
TATGGCG123600.016.21013516
GACGACT68850.016.2052162
TTGACGA71850.016.19605460
TGACGAC73000.015.85880161
ATATGGC229000.015.84773415
AGTGGAC103750.015.7839978
GACGTGG76850.015.6886237
ACGACTT72300.015.51542263
TTAGGAC108850.015.3211473
GACGTGA81650.015.133657
Produced by FastQC (version 0.11.2)