FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13333444
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT333160.2498679260962134Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT19050.061.7324694
CGACGTA25100.048.2068592
CACCGTA26400.044.28809493
TCGACGT28000.043.21399791
ACGTATC28800.040.12535594
ACGCCGT23500.038.47213494
ATGTCGG26450.037.52231692
TGTCGGT25950.036.9355293
GGTCGAC33300.036.540789
GACGTAT32250.035.62209793
TGGTCGA35500.034.08559488
TAGATCG20200.033.3167888
TCACCGT37050.033.025392
GTCGACG37800.032.5498790
TACGAGT15350.031.44216794
GTCGGTG30700.030.9993294
GATGTCG32700.030.97426491
GTGATCG22050.030.5203291
GTGGACG33800.030.36845690
CGGTGGA35350.028.84569788
Produced by FastQC (version 0.11.2)