FastQCFastQC Report
Mon 11 Dec 2017
NZO-M-ATAC-2-12_GTAGAGGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-M-ATAC-2-12_GTAGAGGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13333444
Sequences flagged as poor quality0
Sequence length126
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT334720.251037916385294No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA312920.23468805208916765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG112450.022.035164
GTCCTAC121550.020.3357281
GGACGTG120100.019.3803046
GTAGGAC133550.019.1371973
AGGACGT128100.019.015275
GACGTGG76050.017.1964557
CTACAGT158250.016.7952184
GACGACT69350.016.78085562
TACAGTG161750.016.6914425
TGTAGGA170800.016.649762
CGTCTAG15300.016.4693071
CGTGGAA78950.016.260859
TCCTACA164300.016.1764662
CTGTAGG164000.016.096311
TGACGAC74600.016.08236761
ACGTGGA78500.015.9719618
CGACTTG68450.015.96338564
TTGACGA74050.015.95885160
ACGACTT71750.015.885363
CCTACAG156300.015.814333
Produced by FastQC (version 0.11.2)