FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18579330
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT410580.2209875167726716Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG51300.021.871906120
CGCCGTT30150.021.692097120
TAGGACG93900.020.3219784
CGTATCC131500.019.574646120
TCGCCGG74100.019.352722120
CGGTGGG66750.017.798208120
CCGACGG40750.017.08019120
GGACGTG104700.016.7901746
GTCCTAC126950.016.7803131
TGACGCC53600.016.119816120
CGTCTAG22000.016.0928781
AGGACGT122000.016.0334555
GACGAGG78100.015.13483120
CGTACGG17750.014.53448838
GTAGGAC134800.014.3786143
GTATAGG67400.014.1560351
TACAGTG182850.014.1105145
TATGGCG108900.014.10398116
AGACCGT28400.013.9431186
TTAGGAC114100.013.62133
Produced by FastQC (version 0.11.2)