FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18579330
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA385370.20741867440860354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20450.022.2943861
TAGGACG91850.019.4644244
GGACGTG100250.017.7132786
GTCCTAC129850.017.4170511
CCGTACG16950.017.34125733
GTAGGAC134400.017.2749233
CGTACGT16450.016.77454834
AGGACGT119950.015.9549725
TTGACGG25600.015.46529431
CTACGGT19450.015.11197813
TACAGTG184850.014.4426275
CTAGACG21750.014.3432823
CGAATTG22600.014.06818222
CGTAATC20950.014.03080722
CCTACAG172600.013.9382433
TACACTG157150.013.896095
CTACAGT174850.013.8618174
GGTCGGT36850.013.83668611
TATGGCG111050.013.82918616
TAGACGC43800.013.6971544
Produced by FastQC (version 0.11.2)