FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18555019
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT400190.21567749405160944Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20500.021.368691
TCTCGGG56350.021.189754120
CGTATCC138750.020.757498120
CCGACGG41000.020.049498120
CGCCGTT29600.019.865576120
TCGCCGG76200.019.764486120
TAGGACG94550.018.9766084
GTCCTAC131350.017.6346231
CGGTGGG68750.017.280617120
TTGACGG25050.017.00714731
GACGAGG78450.016.979574120
GGTCGGT40150.015.98927311
GGACGTG106950.015.8761196
GTAGGAC134100.015.2145483
AGGACGT123500.015.012445
TACAGTG181700.014.3315965
CTACGGT18250.014.13631213
TAGATCC159300.014.049467120
CTAGACG22450.013.8993953
CTACAGT172150.013.803754
Produced by FastQC (version 0.11.2)