FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18555019
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA373960.20154115713920856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG21000.025.9958761
TTGACGG26350.018.6678131
TAGGACG94500.018.4113664
GTCCTAC127850.017.314421
GGACGTG103500.015.9975916
CGGCTAT32650.015.98452335
GTAGGAC136200.015.7257343
GTATACG7400.015.4029621
AGGACGT120650.015.01745
CTAGACG20850.014.9629453
ACGGCTA31400.014.90153234
GACGGCT34550.014.75811833
GGTCGGT37000.014.75322311
TTAGACG19450.014.4976643
TAGACGC44650.014.2430894
GCGTAAT17400.014.1356891
GCGAATT20900.014.06558919
GACGACT55600.014.02541762
CGACTTG52950.013.95630264
TACACTG160300.013.8853095
Produced by FastQC (version 0.11.2)