FastQCFastQC Report
Mon 11 Dec 2017
NZO-F-ATAC-2-11_AAGAGGCA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNZO-F-ATAC-2-11_AAGAGGCA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18587526
Sequences flagged as poor quality0
Sequence length126
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT405540.21817857847246588Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG71950.020.763964120
CGTCTAG23000.020.611351
CGGTGGG65600.020.212973120
CGCCGTT29550.020.101082120
CGTATCC144650.019.61934120
TCTCGGG48800.018.688114120
TAGGACG88000.018.5477924
GACGAGG78050.017.526842120
CCGACGG41450.017.225195120
GTCCTAC131500.016.3823381
GGACGTG99900.015.9759696
CCGTACG17750.015.5484119
AGGACGT117050.015.0199455
TTGACGG26650.014.8637631
CGTACGT17350.014.52366910
GTAGGAC136000.014.2517973
CTACAGT175650.014.2460324
TGACGCC53550.014.229382120
TACAGTG188050.013.7523235
CCGTGAT64050.013.399816527
Produced by FastQC (version 0.11.2)